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Beena, V.
- Haematological Responses of Murrah Buffalo Calves to Varying Temperature Humidity Index
Abstract Views :150 |
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Authors
Affiliations
1 Department of Veterinary Physiology, Veterinary College, Mannuthy, Kerala, IN
2 College of Dairy Science, Mannuthy, Kerala, IN
3 University Livestock Farm, Mannuthy, Kerala, IN
1 Department of Veterinary Physiology, Veterinary College, Mannuthy, Kerala, IN
2 College of Dairy Science, Mannuthy, Kerala, IN
3 University Livestock Farm, Mannuthy, Kerala, IN
Source
International Journal of Innovative Research and Development, Vol 5, No 5 (2016), Pagination: 329-331Abstract
The current study was undertaken to investigate the adaptation of Murrah buffalo calves to varying temperature humidity index in the central midlands of Kerala using rectal temperature and haematological parameters. The temperature humidity index recorded throughout the experimental period varied from 76.5 to 80.0. A negative significant correlation was observed between temperature humidity index and haematological parameters (total erythrocyte count, haemoglobin concentration and haematocrit) at P<0.01 level. A significant positive correlation was observed between the temperature humidity index and plasma volume of the experimental animals at P<0.01 level.
Keywords
Temperature Humidity Index, Buffalo, Haematological Parameters, Rectal Temperature, Plasma Volume.Full Text
- Molecular Etiopathology of Naturally Occurring Reproductive Diseases in Female Goats
Abstract Views :136 |
PDF Views:0
Materials and Methods: A total of 660 female genitalia were examined for pathological conditions (macroscopic and microscopic lesions). The etiopathological study was carried out for the presence of pathogenic organisms such as Brucella, Chlamydia, and Campylobacter in the uterus and ovary. Based on the microscopic lesions, suspected samples were subjected to diagnostic polymerase chain reaction (PCR) for various etiological agents employing 16srRNA genus specific primers for Campylobacter and Chlamydophila and OMP31 gene-based PCR for Brucella melitensis and nested PCR using ITS-1 gene primers for Toxoplasma gondii. For Brucella suspected samples, immunohistochemistry (IHC) was also performed.
Results: In studied female genitalia, 108 (16.30%) showed gross abnormalities with overall 23.32% occurrence of pathological conditions (macroscopic and microscopic lesions). Pathological involvement of the uterus was the highest 68 (62.96%), followed by the ovaries 27 (25%) and other organs. Major uterine condition observed was endometritis (5.60%). In uterine infections, 35 (5.30%) samples were found positive for Campylobacter spp., 12 (1.81%) samples for B. melitensis, and 3 (0.45%) samples were positive for Chlamydophila spp. Among the samples positive for B. melitensis by PCR, 3 were found positive by IHC also. Corynebacterium ovis was detected by PCR using specific primers in a case of hydrosalpinx. It was concluded that many pathological lesions in female genitalia of functional significance play a major role in infertility in goats.
Conclusion: The present study concluded that many pathological lesions in female genitalia of functional significance play a major role in infertility in goats.
Authors
V. Beena
1,
R. V. S. Pawaiya
1,
K. Gururaj
1,
D. D. Singh
1,
A. K. Mishra
1,
N. K. Gangwar
1,
V. K. Gupta
2,
R. Singh
3,
A. K. Sharma
3,
M. Karikalan
3,
Ashok Kumar
1
Affiliations
1 Division of Animal Health, ICAR-Central Institute for Research on Goats, Makhdoom, Farah, Mathura-281122, Uttar Pradesh, IN
2 Centre for Animal Disease Research and Diagnosis (CADRAD), ICAR-Indian Veterinary Research Institute, Izatnagar-243122, Uttar Pradesh, IN
3 Division of Pathology, ICAR-Indian Veterinary Research Institute, Izatnagar-243122, Uttar Pradesh, IN
1 Division of Animal Health, ICAR-Central Institute for Research on Goats, Makhdoom, Farah, Mathura-281122, Uttar Pradesh, IN
2 Centre for Animal Disease Research and Diagnosis (CADRAD), ICAR-Indian Veterinary Research Institute, Izatnagar-243122, Uttar Pradesh, IN
3 Division of Pathology, ICAR-Indian Veterinary Research Institute, Izatnagar-243122, Uttar Pradesh, IN
Source
Veterinary World, Vol 10, No 8 (2017), Pagination: 964-972Abstract
Aim: The aim of the present study was to investigate the molecular etiopathology of occurrence of reproductive diseases in female goats. Reproductive diseases in goats account for major economic losses to goat farmers in terms of valuable loss of offspring and animal productivity.Materials and Methods: A total of 660 female genitalia were examined for pathological conditions (macroscopic and microscopic lesions). The etiopathological study was carried out for the presence of pathogenic organisms such as Brucella, Chlamydia, and Campylobacter in the uterus and ovary. Based on the microscopic lesions, suspected samples were subjected to diagnostic polymerase chain reaction (PCR) for various etiological agents employing 16srRNA genus specific primers for Campylobacter and Chlamydophila and OMP31 gene-based PCR for Brucella melitensis and nested PCR using ITS-1 gene primers for Toxoplasma gondii. For Brucella suspected samples, immunohistochemistry (IHC) was also performed.
Results: In studied female genitalia, 108 (16.30%) showed gross abnormalities with overall 23.32% occurrence of pathological conditions (macroscopic and microscopic lesions). Pathological involvement of the uterus was the highest 68 (62.96%), followed by the ovaries 27 (25%) and other organs. Major uterine condition observed was endometritis (5.60%). In uterine infections, 35 (5.30%) samples were found positive for Campylobacter spp., 12 (1.81%) samples for B. melitensis, and 3 (0.45%) samples were positive for Chlamydophila spp. Among the samples positive for B. melitensis by PCR, 3 were found positive by IHC also. Corynebacterium ovis was detected by PCR using specific primers in a case of hydrosalpinx. It was concluded that many pathological lesions in female genitalia of functional significance play a major role in infertility in goats.
Conclusion: The present study concluded that many pathological lesions in female genitalia of functional significance play a major role in infertility in goats.
Keywords
Brucella melitensis, Campylobacter Spp., Chlamydophila Spp., Corynebacterium ovis, Etiopathology, Female Genitalia, Goat, Ovary, Reproductive Diseases, Uterus.Full Text